Dr Alex C Varghese

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Dr Alex C Varghese

Dr. Alex C Varghese received his PhD from University of Calcutta, India for his thesis based on ART. He was a Post Doctoral Research Fellow at the Center for Reproductive Medicine, Cleveland Clinic Foundation, USA from 2007-2008 and then he joined as the Scientific Director and Chief embryologist at the Fertility Clinic, AMRI Hospital, Kolkata, India (2008 to 2011).

In the year 2011 he joined the team of Prof. S.L.Tan at Montreal Reproductive Centre, Quebec, Canada. He is active in clinical embryology since 1997 and has initiated many successful training programs in ART. In the year 2010 he founded the educational web platform for IVF professionals- www.lifeinvitro.com which is now popular in 160 countries. He has authored over 30 manuscripts and edited ten books in IVF, published by Springer, USA, CRC press and Jaypee Medical Publishers. He has also published 17 book chapters in the area of assisted conception. He is also in the editorial board of Journal of Assisted Reproduction, Genetics (ASRM) and International Journal of Infertility and Fetal Medicine, Journal of Reproductive and Stem cell Biotechnology and Obstetrics and Gynecology International.

Dr. Alex’s research interests are in assisted reproductive technology, in particular, in understanding the molecular and environmental causes and prevention of male infertility, genomics and metabolomics in embryology and vitrification of gametes and embryos. He is also the co-investigator in the research project funded by Dept. of Biotechnology, Govt. of India and the current President of Academy of clinical embryologists-India.                                               

                         

 

Books published in 2015

  1. Steven Fleming, Alex C Varghese and James Catt (Edt) A Guide to Organization, Management and Quality Issues in a Tertiary Level IVF Unit, Springer Verlag, USA (In Preparation)
  2. Sandro C Esteves, Alex C Varghese and Kathryn C Worrilow (Edt)  Clean Room Technology in ART Clinics – A Practical Guide, CRC Press (In Preparation)
  3. Zsolts Peter Nagy, Alex C Varghese and Ashok Agarwal (Edt) Gamete & Embryo Cryopreservation- Methods in Molecular Biology Series, Springer Verlag (In Preparation)

Research Publications:

  1. Varghese AC, Goldberg E, Bhattacharyya AK, Agarwal A (2007) Emerging technologies for the molecular study of infertility, and potential clinical applications, Reproductive Biomedicine Online 15, 4, 451-456.
  2. Varghese AC, Goldberg E and Agarwal A (2007) Current and future perspectives on intracytoplasmic sperm injection (ICSI): A critical commentary Reproductive Biomedicine Online 15, 6, 719-727.
  3. Varghese AC, Sinha B, BhattacharyyaAK, New frontiers in the evaluation of sperm function, in vitro selection and manipulation of male gametes for assisted conception, Indian Journal of Experimental Biology, 43, November, 2005, pp.1023-1031
  4. Varghese AC, Stefen du Plessis, Agarwal A (2008) Male Gamete Survival at Stake- causes and solutions, Reproductive Biomedicine Online, 17 (6), 866-880.
  5. Varghese AC, Bragais FM, Mukhopadhyay D, Kundu S, Pal M, Bhattacharyya AK, Agarwal A (2009) Human sperm DNA integrity in normal and abnormal semen samples and its correlation with sperm quality. Andrologia, 41, 207-15.
  6. Bhattacharya, S.M., Mukhopadhyay, D., Varghese, A.C., Kundu, S., Bhattacharyya, A.K. How does oxidative stress affect human sperm function? Embryo Talk, vol. 1 (2006)
  7. Makker K, Varghese AC, Murandi R, Desai N and Agarwal A (2009) Cell phone usage-Modern man’s nemesis, RBM online, 18, 148-157.
  8. Alex C Varghese, Stephen du Plessis, Tomasso Falcone , Ashok Agarwal (2008) Cryopreservation of ovarian tissues and in vitro follicle culture: challenges for fertility preservation Reproductive Biology & Endocrinology, 6, 47
  9. Alex C Varghese, Peter Nagy and Agarwal A (2009) Current trends, biological foundations and future prospects of human oocyte and embryo cryopreservation RBM  Online 19 (1) 126-40
  10. Agarwal A, Desai N, Makker K, Varghese A and Murandi R (2008) Effect of cell phone radiation on sperm function, ROS and DNA integrity: an in vitro study. Fertility and Sterility, doi:10.1016/j.fertnstert.2008.08.022
  11. Varghese AC, Amir Arsalan and Agarwal A (2009) An update on developments and future prospects of ICSI. Arch Med Sci 2009; 5, 1A: S109–S114
  12. Dyutiman Mukhopadhyay, Parag Nandi, , Alex C. Varghese,, Rohit Gutgutia, , Samir Banerjee, , Asok K. Bhattacharyya (2008) The in-vitro effect of benzo-a-pyrene on human sperm hyperactivation and acrosome reaction. Fertility & Sterility (doi:10.1016/j.fertnstert.2009.02.031
  13. Dyutiman Mukhopadhyay, Alex C Varghese, Furquan Pathan, Shukla Kundu, Manisha Pal, Parag Nandi, Samir Banerjee and Asok K Bhattacharyya (2008) Effect of age on semen parameters as observed in 2329 men attending an andrology lab in an Indian city. Human Fertility (communicated)
  14. Kathelene O’Brian, Varghese AC, Agarwal A (2010) Advances in genetics of male infertility. Fertility & Sterility , Jan, (93)3; 1-12
  15. Mukhopadhyay D, Mitra A, Nandi P, Varghese AC, Murmu N, Chowdhury R, Chaudhuri K and Bhattacharyya AK. (2009) Expression of Metallothionein-1 (MT-1) mRNA in the rat testes and liver after Cadmium injection. Syst Biol Reprod Med ;55(5-6):188-92
  16. Mukhopadhyay D, Alex C. Varghese, Manisha Pal, Sudip K. Banerjee, Asok K. Bhattacharyya, Rakesh K. Sharma and Ashok Agarwal, (2009) Semen quality and age-specific changes: A study between two decades on 3729 male partners ofcouples with normal sperm count and attending an andrology laboratory for infertility-related problems in an Indian city. Fertility and Sterility (doi:10.1016/j.fertnstert.2009.01.135 In press)
  17. D. Mukhopadhyay, A. C. Varghese, P. Nandi, S. K. Banerjee & A. K. Bhattacharyya CASA-based sperm kinematics of environmental risk factor-exposed human semen samples designated as normozoospermic in conventional analysis, Andrologia. 2010 Aug;42(4):242-6.
  18. Varghese AC, Kim Ly, Corbin C, Mendiola J and Agarwal A (2010) Oocyte development competence and embryo development: Impact of lifestyle and environment and risk factors, RBM Online 22 (5) 410-420, doi:10.1016/j.rbmo.2010.11.009
  19. Sandro C Esteves, Alex C Varghese (2012) Laboratory handling of epididymal and testicular spermatozoa: what can be done to improve sperm injections outcome J Hum Reprod Sci, 5, 3, 233-243
  20. Ayse Seyhan, Baris Ata, Hai Ying Chen, Alex C. Varghese, Alper Mumcu, Seang Lin Tan Fertility Preservation, Current Obstetrics and Gynecology Reports, December 2012, Volume 1, Issue 4, pp 182-189
  21.  Nicolas Garrido, Alex C Varghese, Rocio M Rivera, Marcos Messeguer, OMICS- current and future perspectives in reproductive medicine and technology, Journal of Human Reproductive Sciences (In Press)

Abstracts in International Meetings: 

  1. Varghese A C, Bhattacharyya A K, Roy H, Allamaneni S S, Bhattacharyya J, Agarwal A. A comparative study of nuclear DNA integrity and morphology of human spermatozoa processed by three different methods. Fertil Steril 82, Sup 2 (2004) S104.
  1. Pal S, Varghese AC, Agarwal A, Bhattacharyya AK, The effect of temperature and duration of cryopreservation on human sperm characteristics. Fertil Steril 82; Sup 2 (2004)
  1. Varghese AC, Chakraborty D, Bhattacharyya AK. Said TM, Bhattacharya SM, Agarwal A. Enhancement of human sperm motility by inclusion of acetyl-L-carnitine in processing media Fertil Steril 84; Sup.1,(2005)S105.
  1. Bhattacharyya AK, Varghese AC, Das S, Biswas A, Mondal M, Agarwal A. Motion characteristics of frozen-thawed human spermatozoa processed by different methods: a comparative study, Fertil Steril 84; Sup.1,(2005)S177.
  1. Varghese AC, Das S, Bhattacharyya AK, Bhattacharya SM, Mondal M, Agarwal A. Effect of cryoprotective additives- reduced glutathione, acetyl-L-carnitine on sperm membrane lipid peroxidation, DNA integrity and recovery of motile human sperm Fertil Steril 84; Sup.1,(2006)S105.
  1. Bhattacharyya, A.K.; Chakraborty, D.; Varghese, A.C.; Bhattacharyya, S.M.; Kundu, S.; Banerjee, A. Cryopreservation and post-thaw motility, DNA integrity and acrosome status of pre-freeze prepared human semen frozen in different trehalose concentrations. Hum. Reprod. 21; Sup.1,(2006)P-393.
  1. Satyasrini, S.V.; Varghese, A.C.; Chakraborty, D.; Kundu, S.; Vasan, B.; Bhattacharyya, A.K. N-acetyl glucosamine and beta glycerophosphate supplementation in cryopreservation media on post thaw sperm functional characteristics. Hum. Reprod. 21; Sup.1,(2006)P-391.
  1. Vasan, B.; Chakraborty, D.; Satyasrini, V.; Varghese, A.C.; Bhattacharya, S.M.; Bhattacharyya, A.K. Dose dependent effect of cryoprotective agents on post thaw human sperm motility, morphology, lipid peroxidation and DNA integrity. Hum. Reprod. 21; Sup.1,(2006)P-390.
  1. J. Bhattacharya, S. Kundu, A. C. Varghese, S. M. Bhattacharya, A. K. Bhattacharyya, A. Agarwal A comparative study on TEST-Yolk buffer and human sperm preservation medium on post thaw characteristics of human sperm from pre-freeze specimens. Fertil. Steril 2006; 86, (3)

 

  1. Bhattacharyya, A. C.Varghese, S. M. Bhattacharya, P. Ray, J. Bhattacharya, A. Agarwal A computer assisted sperm motility based study on rapid vs. slow thawing temperatures on human sperm motility parameters. Fertil. Steril  2006; 86, (3) S197
  1. C. Damayandi, A. C. Varghese, D. Mukhopadhaya, S. M. Bhattacharya,K. Bhattacharyya, A. Agarwal Motility characteristics and DNA integrity of spermatozoa processed with different concentrations of reduced glutathione (GSH) Fertil. Steril 2006; 86, (3) S446
  1. Mosammat Begum, David Miller, M A Salam, Ehsam Quadir, Alex C. Varghese, Ashok Agarwal Antibiotics and micronutritional blend to enhance fertility potential in men with abnormal semen quality (Accepted for presentation at  64th Annual Meeting of the American Society for Reproductive Medicine)
  1. Sajal Gupta, Reda Mahfouz, Alex Varghese, Rakesh Sharma, Edmund Sabanegh, Ashok Agarwal Association of sperm DNA fragmentation with seminal oxidative stress in male factor infertility (Submitted to 64th Annual Meeting of the American Society for Reproductive Medicine)
  1. Israel Maldonaldo, Alexandra Bermudez, Alex Varghese, Jose Gaytan, Rakesh Sharma, Ashok Agarwal Oocyte dysmorphism is not a limiting factor for post-thaw survival following vitrification by cryotop method (Accepted for presentation at  64th Annual Meeting of the American Society for Reproductive Medicine) 
  1. Asok Bhattacharya, S Suresh, V Vineetha, Bijoy Sebastian, Alex Varghese, Ashok Agarwal Antioxidant effects of Triphala, Brahmi and Triphala- Brahmi combination in blood and seminal plasma of high glucose fed rats (Accepted for presentation at  64th Annual Meeting of the American Society for Reproductive Medicine)
  2. Parag Nandi, Alex C Varghese et al A preliminary study on heavy metals (lead, cadmium and arsenic) in follicular fluid and its effect on oocyte, embryo quality and outcome of ivf treatment (Presented at Annual meeting of ASRM, Orlando 2011)
  3. I. Maldonado, A. Varghese  .E. Cervantes . A Góngora . R Sharma . J. Granja . M.T. Márquez  and A. Agarwal . Oocyte morphology quality is not a limiting factor for the  survival processs on ageing oocytes  after vitrification and warming  using   cryotop method. (Accepted for presentation at ESHRE annual meeting Turkey, 2012)

Chapters published in Books 

  1. Alex C Varghese, Damayanti Chakraborty, A. K. Bhattacharyya. (2006) Ovarian tissue cryopreservation, chapter in, Kamini Rao (editor): ART Laboratory manual, Jaypee Medical Publishers
  2. Agarwal A, Vaghese AC, Sharma R (2009) Markers of oxidative stress and sperm chromatin integrity.In: Molecular Endocrinology-Methods & protocols, Park-Sarge, Ok-Kyong; Curry, Jr., Thomas (Eds.) , Humana Press, Springer- Verlag
  3. Makker, K, Varghese, A., Sharma, RK, Agarwal, A. (2008): Interactions between reactive oxygen species and male germ cells. In: Biology of Male Germ Cells (Editors: Hans-Juergen Glander and Uwe Paasch) B, pp 273-310. 4.
  4. Varghese, C., Deepinder, F., Chandra, A., Jeat, A., Pathan, F., Agarwal, A. (2010): Anatomy and physiology of male gametogenesis. In: Andrology Laboratory Manual (Editors: Kamini Rao, Agarwal, A, Srinivas M S) Jaypee Brothers Pvt Ltd, New Delhi. Chapter 1, pp 3-9.
  5. Kundu, S., Bhattacharya, A., Varghese, A., Chakravarty, B., Agarwal, A. (2010): Sperm- cervical mucus interaction test and its importance in the management of infertility. In: Andrology Laboratory Manual (Editors: Kamini Rao, Agarwal, A, Srinivas M S) Jaypee Brothers Pvt Ltd, New Delhi. Chapter 12, pp 122-126.
  6. Varghese, A., Nandi, P., Mahfouz, R., Athayde, K., Agarwal, A. (2010): Human sperm cryopreservation. In: Andrology Laboratory Manual (Editors: Kamini Rao, Agarwal, A, Srinivas M S) Jaypee Brothers Pvt Ltd, New Delhi, Chapter 21, pp 196-208.
  7. Athayde, K., Varghese, A., Agarwal, A. (2010): Quality management of the Andrology laboratory. In: Andrology Laboratory Manual (Editors: Kamini Rao, Agarwal, A, Srinivas M S) Jaypee Brothers Pvt Ltd, New Delhi, Chapter 17, pp 165-172.
  8. Varghese, A., Pathan, F., Jeat, A., Agarwal, A. (2010): Semen analysis. In: Andrology Laboratory Manual (Editors: Kamini Rao, Agarwal, A, Srinivas M S) Jaypee Brothers Pvt Ltd, New Delhi. Chapter 11, pp 107-121.
  9. Alex C. Varghese, C. Fischer–Hammadeh, and M. E. Hammadeh Acridine Orange Test for Assessment of Human Sperm DNA Integrity  in Armand Zini, Agarwal A (edt): Human sperm DNA integrity, Humana Press, Springer- Verlag, USA ( July 2011)
  10. Alex C. Varghese, Eulalia Pozo-Guisado, Ignacio S. Alvarez, and Francisco Javier Martin-Romero Extended Embryo Culture Supplementation in Male Infertility: Contemporary Clinical Approaches, Andrology, ART and Antioxidants Editors: Sijo Parekattil and Ashok Agarwal Springer Science + Business Media, LLC, 2012
  11. Alex C Varghese, Nicholas Prados (2012) IVF Work stations, In Peter Nagy, Alex Varghese, A. Agarwal (edt) PRACTICAL MANUAL OF IN VITRO FERTILIZATION: Newer methods and novel devices, SPINGER VERLAG, USA
  12. Madhab CD, Jayaprakashan K, Sameer Angras, Alex C Varghese (2012) Batch IVF- clinical and laboratory perspectives, In Varghese AC et al (edt) A practical Guide to setting up an IVF lab, embryo culture systems and running the unit..Jaypee Medical Publishers
  13. Alpesh Doshi, Sandeep karunakaran, Katy Worrilow, Alex C Varghese, What makes an IVF lab successful-, In Varghese AC et al (edt) A practical Guide to setting up an IVF lab, embryo culture systems and running the unit..Jaypee Medical Publishers (2012)
  14. Alex C Varghese, Seang Lin Tan et al (2012) In vitro maturation of oocytes for fertility preservation and treatment, In Prof John Studd, Prof Seang Lin Tan and Prof. Frank Chervenak (edt) Current Progress in Obstetrics and Gynecology, Kothari Medical Subscription Services (In press)
  15. Haifa Honeneih, Kannamannadiar Jayaprakasan, Alex C Varghese, In Vitro Fertilization and Embryo Transfer in Vol II-IVF and Laboratory Aspects of Andrology in Kamini Rao (Edt) Principles and Practice of Assisted Reproductive Technology, Jaypee Brothers, 2013 http://www.jaypeebrothers.com/pgDetails.aspx?book_id=9789350907368
  16. Alex C Varghese, Giles Palmer, Intracytoplasmic Sperm Injection in Vol II-IVF and Laboratory Aspects of Andrology in Kamini Rao (Edt) Principles and Practice of Assisted Reproductive Technology, Jaypee Brothers, 2013 http://www.jaypeebrothers.com/pgDetails.aspx?book_id=9789350907368
  17. Balaji Prasath, Amr Kader, Sujatha R, Alex C Varghese, Ovarian Tissue Cryopreservation in Vol II-IVF and Laboratory Aspects of Andrology in Kamini Rao (Edt) Principles and Practice of Assisted Reproductive Technology, Jaypee Brothers, 2013 http://www.jaypeebrothers.com/pgDetails.aspx?book_id=9789350907368
  18. Pere Mir, Belen Aparicio, Rubio Carmen, Marcos Meseguer and Alex C Varghese, Embryo Selection: Non invasive and Invasive, In  Gautam Allahabadia (edt) The Art and Science of Assisted reproductive Technology (In press)